161. The conversion of L-glutamate to a-oxoglutarate is catalyzed by the enzyme glutamate dehydrogenase (HDH): L - GDH glutamate → a-oxoglutarate HDH H NH3. At different substrate concentrations, the following initial reaction rates were obtained: [S], mmol/l 1.68 3.33 5.00 6.67 10.00 20.00
v, mmol/l*min 0.172 0.250 0.286 0.303 0.334 0.384
Determine Km, vmax and aE if the enzyme concentration in all cases is constant and equal to 10-5 mol/l

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