71. Solving a chemistry problem
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10. Determine the values of the Michaelis constant and the maximum rate for the hydrolysis of N-acetyl-L-valine methyl ester catalyzed by α-chymotrypsin. Experimental data on the dependence of the initial velocity on the substrate concentration:
[S]0, M r0∙106, M∙s-1
0.200 4.57
0.124 3.83
0.124 3.84
0.091 3.33
0.091 3.31
0.071 2.97
0.071 2.93
0.060 2.67
0.060 2.74
[S]0, M r0∙106, M∙s-1
0.200 4.57
0.124 3.83
0.124 3.84
0.091 3.33
0.091 3.31
0.071 2.97
0.071 2.93
0.060 2.67
0.060 2.74
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